Journal: Respiratory Research

Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

doi: 10.1186/s12931-018-0768-1

Figure Lengend Snippet: Primer table for Real-Time Quantitative Reverse-Transcriptase PCR (qRT-PCR). Primers were synthesized by MWG Eurofins (Ebersberg, Germany).

Article Snippet: Integrin-β1 , ITGB1 , mouse monoclonal anti-ITGB1 antibody , Abcam, Cambridge, UK , WB.

Techniques: Synthesized

Journal: Respiratory Research

Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

doi: 10.1186/s12931-018-0768-1

Figure Lengend Snippet: Primary antibodies used in Western Blot analysis, Immunofluorescence and Proximity Ligation Assays

Article Snippet: Integrin-β1 , ITGB1 , mouse monoclonal anti-ITGB1 antibody , Abcam, Cambridge, UK , WB.

Techniques: Western Blot, Immunofluorescence, Ligation

Journal: Respiratory Research

Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

doi: 10.1186/s12931-018-0768-1

Figure Lengend Snippet: FKBP10 deficiency alters the expression of molecules implicated in adhesion and migration. a, c, e, g, i Western Blot analysis of phLF treated with scrambled siRNA as control (sc) or FKBP10 siRNA (kd) and 2- phosphoascorbate (0.1 mM) in absence and presence of TGF-β1 (2 ng/mL) for 48h. Densitometric analysis and representative blots show the effect of FKBP10 kd on the expression of talin-1 (TLN1) ( a ), calpain-4 (CAPNS1) ( c ), integrin β1 (ITGB1) ( e ), caveolin-1 (CAV1) ( g ) and coronin 1C (CORO1C) ( i ) relative to β-actin as loading control (ACTB). b, d, f, h, j Quantitative reverse transcriptase-polymerase chain reaction analysis of phLF treated with scrambled siRNA as control (sc) or FKBP10 siRNA (kd) and 2- phosphoascorbate (0.1 mM) in absence and presence of TGF-β1 (2 ng/mL) for 48h. Transcript levels are presented as -ΔCt values showing the effect of siRNA mediated kd of FKBP10 on talin-1 (TLN1) ( b ), calpain-4 (CAPNS1) ( d ), integrin β1 (ITGB1) ( f ), caveolin-1 (CAV1) ( h ) and coronin 1C (CORO1C) ( j ). DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 (DHX8) was used as endogenous control. All data is based on eight (protein) or seven (mRNA) independent experiments. Statistical significance between control and FKBP10 kd is indicated by horizontal brackets and asterisks

Article Snippet: Integrin-β1 , ITGB1 , mouse monoclonal anti-ITGB1 antibody , Abcam, Cambridge, UK , WB.

Techniques: Expressing, Migration, Western Blot, Polymerase Chain Reaction