Journal: Respiratory Research

Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

doi: 10.1186/s12931-018-0768-1

Figure Lengend Snippet: Primer table for Real-Time Quantitative Reverse-Transcriptase PCR (qRT-PCR). Primers were synthesized by MWG Eurofins (Ebersberg, Germany).

Article Snippet: Integrin-β1 , ITGB1 , mouse monoclonal anti-ITGB1 antibody , Abcam, Cambridge, UK , WB.

Techniques: Reverse Transcription, Synthesized

Journal: Respiratory Research

Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

doi: 10.1186/s12931-018-0768-1

Figure Lengend Snippet: Primary antibodies used in Western Blot analysis, Immunofluorescence and Proximity Ligation Assays

Article Snippet: Integrin-β1 , ITGB1 , mouse monoclonal anti-ITGB1 antibody , Abcam, Cambridge, UK , WB.

Techniques: Western Blot, Immunofluorescence, Ligation, Control

Journal: Respiratory Research

Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis

doi: 10.1186/s12931-018-0768-1

Figure Lengend Snippet: FKBP10 deficiency alters the expression of molecules implicated in adhesion and migration. a, c, e, g, i Western Blot analysis of phLF treated with scrambled siRNA as control (sc) or FKBP10 siRNA (kd) and 2- phosphoascorbate (0.1 mM) in absence and presence of TGF-β1 (2 ng/mL) for 48h. Densitometric analysis and representative blots show the effect of FKBP10 kd on the expression of talin-1 (TLN1) ( a ), calpain-4 (CAPNS1) ( c ), integrin β1 (ITGB1) ( e ), caveolin-1 (CAV1) ( g ) and coronin 1C (CORO1C) ( i ) relative to β-actin as loading control (ACTB). b, d, f, h, j Quantitative reverse transcriptase-polymerase chain reaction analysis of phLF treated with scrambled siRNA as control (sc) or FKBP10 siRNA (kd) and 2- phosphoascorbate (0.1 mM) in absence and presence of TGF-β1 (2 ng/mL) for 48h. Transcript levels are presented as -ΔCt values showing the effect of siRNA mediated kd of FKBP10 on talin-1 (TLN1) ( b ), calpain-4 (CAPNS1) ( d ), integrin β1 (ITGB1) ( f ), caveolin-1 (CAV1) ( h ) and coronin 1C (CORO1C) ( j ). DEAH (Asp-Glu-Ala-His) Box Polypeptide 8 (DHX8) was used as endogenous control. All data is based on eight (protein) or seven (mRNA) independent experiments. Statistical significance between control and FKBP10 kd is indicated by horizontal brackets and asterisks

Article Snippet: Integrin-β1 , ITGB1 , mouse monoclonal anti-ITGB1 antibody , Abcam, Cambridge, UK , WB.

Techniques: Expressing, Migration, Western Blot, Control, Reverse Transcription, Polymerase Chain Reaction

Journal: Journal of Nanobiotechnology

Article Title: Increased BMSC exosomal miR-140-3p alleviates bone degradation and promotes bone restoration by targeting Plxnb1 in diabetic rats

doi: 10.1186/s12951-022-01267-2

Figure Lengend Snippet: Histological/Immunohistological analysis of bone formation after transplantation of exosomes. A – C Histological analysis of decalcified sections in both normal and diabetic rats. HE/Masson’s/ Safranin O-fast green staining of new bone formation in the bone defect. The black arrow indicates new bone in light pink, blue, or green within the circle, indicating the 3 mm-diameter defect. New bone formation in the bone defects was analyzed using ImageJ software. n = 5 for each group. D – F Immunohistological analysis of the osteoblasts and BMSCs in both normal and diabetic rats. Collagen-1 and CD29/Sca-1 staining of osteoblasts and BMSCs in bone defects. Immunohistochemical staining with IgG was as the control group. Black arrows indicate osteoblasts and BMSCs on the bone surface. n = 5 in each group; *p < 0.05; **p < 0.01; ***p < 0.001. Data represent means ± SD

Article Snippet: Finally, the samples were incubated with a specific primary antibody for 12 h at 4 °C, from the following panel: mouse monoclonal anti-collagen I (Col-1;ab270993, Abcam); rabbit polyclonal anti-CD29 (GeneTex,California,USA,GTX128839); rabbit anti-mouse Sca-1 (Biolegend, California,USA,108,103) and Rabbit IgG(ab125938) which was regarded as negative control in IHC.

Techniques: Transplantation Assay, Staining, Software, Immunohistochemical staining

Journal: Journal of Nanobiotechnology

Article Title: Increased BMSC exosomal miR-140-3p alleviates bone degradation and promotes bone restoration by targeting Plxnb1 in diabetic rats

doi: 10.1186/s12951-022-01267-2

Figure Lengend Snippet: MiR-140-3p promotes bone formation in DM rats with bone defects. A Representative micro-CT images within a region of interest (ROI) comprising the bone defect after 2 and 8 weeks following surgery in the bone defect of normal rats and those with diabetes mellitus after treatment with N-Exos, DM-Exos, and Exos overexpressing miR-140-3p . n = 5 in each group. B Quantitative analysis of new bone formation from micro-CT imaging in DM rats with bone defects after 2 and 8 weeks after transplantation with N-Exos, DM-Exos, or Exos overexpressing miR-140-3p . BV: bone volume; TV: tissue volume; Tb,N: trabecular number; Tb.Th: trabecular thickness; Tb.Sp: trabecular Spacing. n = 5 in each group. C – E Histological analysis of decalcified sections of bone defects in diabetic rats treated with N-Exos, DM-Exos, or Exos overexpressing miR-140-3p . HE/Masson’s/Safranin O/fast green staining of the new bone formation within the defect. Black arrows indicate new bone stained in light pink/blue/green within the 3 mm-diameter defect marked as a circle. Quantitative analysis of new bone formation was performed using ImageJ software. n = 5 in each group. F – H Immunohistological analysis of osteoblasts and BMSCs in diabetic rats treated with N-Exos, DM-Exos, or Exos overexpressing miR-140-3p . Collagen-1 and CD29/Sca-1 staining of osteoblasts and BMSCs in sections containing the bone defect. Black arrows indicate osteoblasts and BMSCs on the bone surface. n = 5 in each group; *p < 0.05; **p < 0.01; ***p < 0.001. Data represent means ± SD

Article Snippet: Finally, the samples were incubated with a specific primary antibody for 12 h at 4 °C, from the following panel: mouse monoclonal anti-collagen I (Col-1;ab270993, Abcam); rabbit polyclonal anti-CD29 (GeneTex,California,USA,GTX128839); rabbit anti-mouse Sca-1 (Biolegend, California,USA,108,103) and Rabbit IgG(ab125938) which was regarded as negative control in IHC.

Techniques: Micro-CT, Imaging, Transplantation Assay, Staining, Software